Pathway Studio enables analysis and visualization of disease mechanisms, gene expression and proteomics and metabolomics data. It is an exhaustive resource of easily searchable data from biology articles describing interactions between molecules, cell processes, and diseases. It also helps biological researchers import and analyze their experimental data using statistical tools. Browsing its expertly curated signaling pathway collection lets researchers put to use network and pathways analysis for modeling the impact of differential gene or protein expression and protein-protein interactions on disease, streamlining the involved biological process in drug target discovery. Visualizing free interactive signaling pathway samples from curated database outlines major trends in biological research of signal transduction pathways.